Proteomics

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Structure and proteomics on centrosome from differentiated and quiescent cells: the thymocyte case

ABSTRACT: We have applied cryo-tomography and proteomics analyses to centrosomes isolated from young lamb thymus– a convenient source of quiescent and differentiated cells. Those analyses have allow us to better depict the protein organization joining the two centrioles in such a cellular system, in the form of a disperse network of fibers and an amorphous density, both parts linked together. Protein composition of the centrosome have been compared with published data on centrosome extracted from cell culture , and presence of various protein reported implicated in the disengagement and dissociation processes questioned. C-NAP1, cohesin smc1 and furthermore condensin smc4 and ncapd2 have been localized on the amorphous density, however DNA hasn't. Subtomogram averaging processing have been applied to the centriolar wall, both on the proximal microtubule triplets and distal doublets; results have been compared to the already published structures of centriolar wall triplet from distant species basal bodies.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Ovis Aries

TISSUE(S): Thymocyte, Thymus

SUBMITTER: Adán Alpízar  

LAB HEAD: Jose Maria Carazo

PROVIDER: PXD003928 | Pride | 2019-11-08

REPOSITORIES: Pride

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Publications

Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes.

Busselez Johan J   Chichón Francisco Javier FJ   Rodríguez Maria Josefa MJ   Alpízar Adan A   Gharbi Séverine Isabelle SI   Franch Mònica M   Melero Roberto R   Paradela Alberto A   Carrascosa José L JL   Carazo José-Maria JM  

Scientific reports 20190510 1

We have used cryo Electron Tomography, proteomics and immunolabeling to study centrosomes isolated from the young lamb thymus, an efficient source of quiescent differentiated cells. We compared the proteome of thymocyte centrosomes to data published for KE37 cells, focusing on proteins associated with centriole disengagement and centrosome separation. The data obtained enhances our understanding of the protein system joining the centrioles, a system comprised of a branched network of fibers link  ...[more]

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