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An omics-based metabolic model of methane-driven denitrification by aerobic methanotrophic bacteria

ABSTRACT: Methylomonas denitrificans is the first characterized organism that couples methane oxidation to nitrate reduction in pure culture without a partner. The aim of this study is to develop a metabolic model of methane driven nitrate respiration using a multi-level omics approach. Samples of M. denitrificans biomass were harvested during growth at high and limiting oxygen and whole transcriptome/proteome analyses were carried out on samples from both conditions. Comparative analysis of transcript and protein abundance between the two conditions was used to develop a metabolic model of methane driven nitrate respiration. 1. Wisniewski JR, Zougman A, Nagaraj N, Mann M: Universal sample preparation method for proteome analysis. Nat Meth 2009, 6:359-362. 2. Oberg AL, Vitek O: Statistical design of quantitative mass spectrometry-based proteomic experiments. J Proteome Res 2009, 8:2144-2156. 3. Olsen JV, de Godoy LMF, Li G, Macek B, Mortensen P, Pesch R, Makarov A, Lange O, Horning S, Mann M: Parts per million mass accuracy on an orbitrap mass spectrometer via lock mass injection into a C-trap. Mol Cell Proteomics 2005, 4:2010-2021.

INSTRUMENT(S):

ORGANISM(S): Methylomonas Denitrificans

SUBMITTER: Manuel Kleiner

LAB HEAD: Manuel Kleiner

PROVIDER: PXD004041 | Pride | 2025-09-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	800ng_1.msf	Msf
	800ng_1.pdResult	Other
	800ng_1.raw	Raw
	800ng_10.msf	Msf
	800ng_10.pdResult	Other

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