Bathymodiolus azoricus host-symbiont proteomics

ABSTRACT: Bathymodiolus azoricus is a deep-sea mussel found in the hydrothermal vent fields of the Mid-Atlantic Ridge. It lives in symbiosis with sulfur- and methane-oxidizing γ-proteobacteria within its gills. In our study, we aimed to understand the metabolic and physiological interconnections between the symbiotic partners. For this purpose, symbionts and host were physically separated using density gradient centrifugation. This procedure yielded a symbiont-enriched gradient pellet fraction and a supernatant fraction enriched in host components. The cytosolic and membrane-associated proteome of both these fractions along with whole gill and foot tissue of the mussel were then investigated through 1D-PAGE LC-MS/MS. Proteins were quantified based on their spectral counts using the NSAF method. For efficient identification, sequences from evolutionarily related endosymbiotic and free-living bacteria and from bivalve host relatives were compiled into a comprehensive protein database. A total of 3178 host and symbiont proteins were identified from all samples.

OTHER RELATED OMICS DATASETS IN: PRJEB8263 PRJEB7941 PRJEB7943 PRJEB8264 PRJEB13047

INSTRUMENT(S):

ORGANISM(S): Thiotrophic Endosymbiont Of Bathymodiolus Azoricus (menez Gwen) Methanotrophic Endosymbiont Of Bathymodiolus Azoricus (menez Gwen) Bathymodiolus Azoricus (mussel)

TISSUE(S): Gill, Foot Muscle

SUBMITTER: Ruby Ponnudurai  

LAB HEAD: Thomas Schweder, Stephanie Markert

PROVIDER: PXD004061 | Pride | 2016-10-06

REPOSITORIES: Pride