Proteomics

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Spatiotemporal proteomic profiling of cerebral development

ABSTRACT: iPS cell based neuronal differentiation was used to generate pure NPCs and maturing nerons in tissue culture. LFQ mass spectrometry on Q Exactive was used to measure proteomic profile differences between the generated cell types. The identified proteomic signatures were then compared to human fetal cerebral development over the developmental age of 16-36 gestational weeks in four macrodissected regions. Statistical analysis were performed using the Perseus program to compare the datasets and identify spatiotemporally relevant protein modules.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neuron, Cell Culture

SUBMITTER: Ugljesa Djuric  

LAB HEAD: Eleftherios Diamandis

PROVIDER: PXD004075 | Pride | 2017-07-14

REPOSITORIES: Pride

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Spatiotemporal Proteomic Profiling of Human Cerebral Development.

Djuric Ugljesa U   Rodrigues Deivid C DC   Batruch Ihor I   Ellis James J   Shannon Patrick P   Diamandis Phedias P  

Molecular & cellular proteomics : MCP 20170707 9

Mass spectrometry (MS) analysis of human post-mortem central nervous system (CNS) tissue and induced pluripotent stem cell (iPSC)-based directed differentiations offer complementary avenues to define protein signatures of neurodevelopment. Methodological improvements of formalin-fixed, paraffin-embedded (FFPE) protein isolation now enable widespread proteomic analysis of well-annotated archival tissue samples in the context of development and disease. Here, we utilize a shotgun label-free quanti  ...[more]

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