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Gulf toadfish intestinal fluid and intestinal CaCO3 matrix proteome

ABSTRACT: Marine teleosts produce CaCO3 in their intestine as part of their osmoregulatory strategy. This study aimed to characterize the proteinaceous matrix associated with these precipitates and compare its constituents to the proteins found in the intestinal fluid.

INSTRUMENT(S):

ORGANISM(S): Opsanus Beta

TISSUE(S): Intestinal Juice

SUBMITTER: Kevin Schauer

LAB HEAD: Martin Grosell

PROVIDER: PXD004135 | Pride | 2016-09-29

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	IF_2 (F020265).mzid_IF_2_(F020265).pride.mgf.gz	Mzid
	IF_2 (F020265).pride.mztab.gz	Mztab
	IF_2.raw	Raw
	IF_2F020265.mzid.gz	Mzid
	IF_2F020265.mzid_IF_2_F020265.MGF	Mzid

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Publications

A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine.

Schauer Kevin L KL LeMoine Christophe M R CM Pelin Adrian A Corradi Nicolas N Warren Wesley C WC Grosell Martin M McDonald M Danielle MD

Scientific reports 20161003

Marine teleost fish produce CaCO<sub>3</sub> in their intestine as part of their osmoregulatory strategy. This precipitation is critical for rehydration and survival of the largest vertebrate group on earth, yet the molecular mechanisms that regulate this reaction are unknown. Here, we isolate and characterize an organic matrix associated with the intestinal precipitates produced by Gulf toadfish (Opsanus beta). Toadfish precipitates were purified using two different methods, and the associated ...[more]

PMID: 27694946

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Analysis of Gulf Toadfish Intestinal Precipitate Matrix

Project description:Marine teleosts produce CaCO3 in their intestine as part of their osmoregulatory strategy. This study aimed to characterize the proteinaceous matrix associated with these precipitates.

2016-10-05 | PXD004134 | Pride

Analysis of the Gulf toadfish intestinal proteome response to chronic hypersalinity exposure

Project description:The marine teleost intestine plays a vital role in whole body salt and water homeostasis. Marine fish must drink seawater in order to rehydrate, and processing of that seawater throughout the gastrointestinal tract allows for the extraction of water from this highly hyperosmotic source. Although the molecular mechanisms of this process have been the subject of much investigation, numerous questions remain. Here, Gulf toadfish (Opsanus beta) were acclimated to normal seawater (35 ppt) of hypersaline seawater (60 ppt) and changes in the anterior intestine, posterior intestine, and intestinal fluid proteomes were investigated using a shotgun proteomics approach employing isobaric TMT tags.

2018-06-26 | PXD005923 | Pride

Rescue of chloride and bicarbonate transport by elexacaftor-ivacaftor-tezacaftor in organoid-derived CF intestinal and cholangiocyte monolayers

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Gene expression timecourse in zebrafish whole eye in response to optic nerve crush

Project description:It is well-established that neurons in the adult mammalian central nervous system are terminally differentiated and, if injured, will be unable to regenerate their connections. In contrast to mammals, zebrafish and other teleosts display a robust neuroregenerative response. Following optic nerve crush (ONX), retinal ganglion cells (RGC) regrow their axons to synapse with topographically correct targets in the optic tectum, such that vision is restored in ~21 days. What accounts for these differences between teleostean and mammalian responses to neural injury is not fully understood. A time course analysis of global gene expression patterns in the zebrafish eye after optic nerve crush can help to elucidate cellular and molecular mechanisms that contribute to a successful neuroregeneration. We used microarrays to detail the global gene expression patterns underlying a successful regeneration or the optic nerve following injury. Experiment Overall Design: Microarray analysis was performed on total RNA extracted from whole eye following optic nerve crush (ONX) or sham surgery at defined intervals (4, 12, & 21 days).

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Calcium effect to the Clostridium beijerinckii NCIMB 8052 degenerate strain for the solvent producing recovery

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Resistant response of leaves of upland cotton line Im216 to Xanthomonas campestris pv. malvacearum race 1

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