Proteomics

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Analysis of the Gulf toadfish intestinal proteome response to chronic hypersalinity exposure

ABSTRACT: The marine teleost intestine plays a vital role in whole body salt and water homeostasis. Marine fish must drink seawater in order to rehydrate, and processing of that seawater throughout the gastrointestinal tract allows for the extraction of water from this highly hyperosmotic source. Although the molecular mechanisms of this process have been the subject of much investigation, numerous questions remain. Here, Gulf toadfish (Opsanus beta) were acclimated to normal seawater (35 ppt) of hypersaline seawater (60 ppt) and changes in the anterior intestine, posterior intestine, and intestinal fluid proteomes were investigated using a shotgun proteomics approach employing isobaric TMT tags.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Opsanus Beta

TISSUE(S): Intestinal Juice, Intestinal Epithelium, Small Intestine Epithelium

SUBMITTER: Kevin Schauer  

LAB HEAD: Martin Grosell

PROVIDER: PXD005923 | Pride | 2018-06-26

REPOSITORIES: Pride

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Publications

Interrogation of the Gulf toadfish intestinal proteome response to hypersalinity exposure provides insights into osmoregulatory mechanisms and regulation of carbonate mineral precipitation.

Schauer Kevin L KL   Reddam Aalekhya A   Xu Elvis Genbo EG   Wolfe Lisa M LM   Grosell Martin M  

Comparative biochemistry and physiology. Part D, Genomics & proteomics 20180623

Marine bony fish live in a hyperosmotic environment and maintain osmotic homeostasis by drinking seawater, and absorbing salt and water across their gastrointestinal tract. Although the ion and water transport mechanisms in the intestine have been the subject of much study, numerous questions remain unanswered. To address some of these questions, a shotgun proteomics methodology employing isobaric tandem mass tags (TMT) was used to interrogate the anterior intestine, posterior intestine, and int  ...[more]

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