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Adaptive response of the sea urchin Paracentrotus lividus to ocean acidification at a volcanic CO2 vent’s system (Mediterranean Sea, Italy)

ABSTRACT: Ocean acidification is recognized as one of the most pervasive anthropogenic impact on marine life. A variety of responses have been highlighted in different marine organisms ranging from physiology to gene and protein expression. However, most of these studies have been performed in laboratory exposing adults or developmental stages to CO2 enriched seawater. To what extend the information obtained from these in vitro experiments may be extrapolated to a natural environment is questionable. Here, we utilized the Castello volcanic CO2 vents at Ischia as natural laboratory to study the P. lividus population living at the low pH zone (pH~7.8) compared to those of sea urchins living at control sites. Wide-ranging analyses were performed in animals collected at the acidified site, including the monitoring of their position, the determination of the physico-chemical parameters of the coelomic fluid and an in depth characterization of coelomocytes regarding the number and type of cells, Hsp70 expression, redox status and protein expression through de-novo sequencing analyses. In addition, the respiration, nitrogen metabolism, and skeletal mineralogy of urchins from the vent were examined in comparison with those from control animals. Overall these analyses allowed to understand how the sea urchins can thrive in low pH/high CO2.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Paracentrotus Lividus (common Sea Urchin)

TISSUE(S): Coelomic Fluid

SUBMITTER: Simona Nonnis

LAB HEAD: Gabriella Tedeschi

PROVIDER: PXD009395 | Pride | 2022-02-28

REPOSITORIES: Pride

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