Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression during hyper and hypoosmotic stress adaptation in Gillichthys mirabilis


ABSTRACT: This study investigates changes in gene expression associated with adaptation to acute hyper- and hypoosmotic stress in Gillichthys mirabilis gill tissue. Gene expression data was captured as a time course (1, 2, 4, 12 hours past exposure) during the first 12 hours following abrupt transfer to osmotic stress media. We employed a custom made Gillichthys mirabilis cDNA microarray. A total of 39 arrays were used in this study: 12 hyperosmotic stress samples, 12 hypoosmotic stress samples, 12 control samples, and 3 time=0 samples. Three individuals were hybridized at each of the four time points (1, 2, 4, 12 hours past exposure) for experimental and control fish (giving a total of 12). All samples were hybridized against a common reference sample containing pooled cDNAs from several individuals.

ORGANISM(S): Gillichthys mirabilis

SUBMITTER: Tyler Evans 

PROVIDER: E-GEOD-11700 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A microarray-based transcriptomic time-course of hyper- and hypo-osmotic stress signaling events in the euryhaline fish Gillichthys mirabilis: osmosensors to effectors.

Evans Tyler G TG   Somero George N GN  

The Journal of experimental biology 20081101 Pt 22


Cells respond to changes in osmolality with compensatory adaptations that re-establish ion homeostasis and repair disturbed aspects of cell structure and function. These physiological processes are highly complex, and require the coordinated activities of osmosensing, signal transducing and effector molecules. Although the critical role of effector proteins such as Na+, K+-ATPases and Na+/K+/Cl(-) co-transporters during osmotic stress are well established, comparatively little information is ava  ...[more]

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