Insight into enzymatic degradation of corn, wheat, and soybean cell wall cellulose using quantitative secretome analysis of Aspergillus fumigatus

ABSTRACT: Aspergillus fumigatus, a saprophytic filamentous fungus, is known to harbor 263 glycoside hydrolase (GH) encoding genes, suggesting that A. fumigatus is an efficient lignocellulose degrader. Hence the present study uses corn, wheat or soybean as a sole carbon source to culture A. fumigatus under animal physiological condition as to understand how cellulolytic enzymes work together to achieve an efficient degradation of lignocellulose. Using isobaric tags for relative and absolute quantification (iTRAQ) approach, total of ~600 extracellular proteins were identified and quantified, in which ~50 proteins were involved in lignocellulolysis, including cellulases, hemicellulases, lignin-degrading enzymes and some hypothetical proteins.Functional classification of identified proteins showed 15% cellulases, 14 % hydrolases acting on glycosyl bond, and 22% hemicellulases。The approach of highly sensitive quantitative analysis of proteome and transcriptome respond to the probable enzyme combination required in agro industries and animal feeding purpose towards more efficient cellulose degradation and therefore better digestion of intracellular starch. Furthermore, number of important GH family protein, some CBM and polysaccharide lyase family proteins have been identified, which could attribute towards better degradation of lignocelluloses to release starch.

INSTRUMENT(S):

ORGANISM(S): Aspergillus Fumigatus Af293

TISSUE(S): Protein

SUBMITTER: Prakriti Sharma  

LAB HEAD: Cheng Jin

PROVIDER: PXD004670 | Pride | 2016-09-29

REPOSITORIES: Pride