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Proteomic profiling of ectodermal basal disc cells secretion of Hydra

ABSTRACT: Biological adhesion (bioadhesion) is referred to attachment of organisms to either biotic or abiotic surfaces. The differentiated ectodermal basal disc cells of the freshwater cnidarian Hydra secrete proteinaceous glue to temporarily attach themselves to surfaces underwater. In this study, we investigate for the first time the protein content of adhesive secretions from the freshwater cnidarian Hydra magnipapillata strain 105. This secretome were analysed using mass spectrometry and resulting MS/MS data were searched against in silico translated H. magnipapillata transcriptome and results from gene expression.

OTHER RELATED OMICS DATASETS IN: PRJNA324754

INSTRUMENT(S): Q Exactive

ORGANISM(S): Hydra Vulgaris

TISSUE(S): Secretion

SUBMITTER: Marcelo Rodrigues

LAB HEAD: Peter Ladurner

PROVIDER: PXD004899 | Pride | 2017-08-18

REPOSITORIES: Pride

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Profiling of adhesive-related genes in the freshwater cnidarian Hydra magnipapillata by transcriptomics and proteomics.

Rodrigues Marcelo M Ostermann Thomas T Kremeser Leopold L Lindner Herbert H Beisel Christian C Berezikov Eugene E Hobmayer Bert B Ladurner Peter P

Biofouling 20161001 9

The differentiated ectodermal basal disc cells of the freshwater cnidarian Hydra secrete proteinaceous glue to temporarily attach themselves to underwater surfaces. Using transcriptome sequencing and a basal disc-specific RNA-seq combined with in situ hybridisation a highly specific set of candidate adhesive genes was identified. A de novo transcriptome assembly of 55,849 transcripts (>200 bp) was generated using paired-end and single reads from Illumina libraries constructed from different poly ...[more]

PMID: 27661452

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