Proteomics

Dataset Information

63

Quantitative Myogenesis C2C12 Proteome with Dimethyl-Labelling


ABSTRACT: The Z-disc is a protein-rich structure critically important for myofibril development and integrity. In order to monitor the quantiative changes in C2C12 myoblast during myogenesis, a quantitative dimethyl-labelling approach was performed with d0 myoblasts, d5 myotubes and electrical puls stimulated d5 myotubes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus musculus  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Friedel Drepper  

LAB HEAD: Bettina Warscheid

PROVIDER: PXD004960 | Pride | 2017-01-02

REPOSITORIES: Pride

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Publications

Myofibrillar Z-discs Are a Protein Phosphorylation Hot Spot with Protein Kinase C (PKCα) Modulating Protein Dynamics.

Reimann Lena L   Wiese Heike H   Leber Yvonne Y   Schwäble Anja N AN   Fricke Anna L AL   Rohland Anne A   Knapp Bettina B   Peikert Christian D CD   Drepper Friedel F   van der Ven Peter F M PF   Radziwill Gerald G   Fürst Dieter O DO   Warscheid Bettina B  

Molecular & cellular proteomics : MCP 20161227 3


The Z-disc is a protein-rich structure critically important for the development and integrity of myofibrils, which are the contractile organelles of cross-striated muscle cells. We here used mouse C2C12 myoblast, which were differentiated into myotubes, followed by electrical pulse stimulation (EPS) to generate contracting myotubes comprising mature Z-discs. Using a quantitative proteomics approach, we found significant changes in the relative abundance of 387 proteins in myoblasts versus differ  ...[more]

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