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Analysis of the Babesia canis secreted proteome during virulent infection

ABSTRACT: Secreted parasite factors from Babesia canis during acute infection were investigated using a LC-MS/MS assay which led to the discovery of potential pathogenicity factors. Combined data from genomics, transcriptomics and proteomics provide a first predicted and partially validated set of potential virulence factors exported during fatal infections.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Babesia Canis Canis Babesia Canis

TISSUE(S): Culture Supernatant, Eukaryotic Cell, Infected Cell, Blood

DISEASE(S): Babesiosis

SUBMITTER: Ramon Eichenberger

LAB HEAD: Adrian B. Hehl

PROVIDER: PXD005117 | Pride | 2017-06-16

REPOSITORIES: Pride

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Genome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factors.

Eichenberger Ramon M RM Ramakrishnan Chandra C Russo Giancarlo G Deplazes Peter P Hehl Adrian B AB

Scientific reports 20170613 1

Infections of dogs with virulent strains of Babesia canis are characterized by rapid onset and high mortality, comparable to complicated human malaria. As in other apicomplexan parasites, most Babesia virulence factors responsible for survival and pathogenicity are secreted to the host cell surface and beyond where they remodel and biochemically modify the infected cell interacting with host proteins in a very specific manner. Here, we investigated factors secreted by B. canis during acute infec ...[more]

PMID: 28611446

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