Dataset Information

Salinity-induced Palmella Formation Mechanism in Dunaliella salina Revealed by Phosphoproteomics

ABSTRACT: Palmella stage is critical for some unicellular algae to survive in extreme environment conditions. The halotolerant algae Dunaliella salina is a good single-cell model for studying plant adaptation to high salinity. We found 35 salinity-responsive phosphoproteins were involved in multiple signaling and metabolic pathways upon palmella formation. The patterns of protein accumulation exhibited changes, including dynamics of cytoskeleton and cell membrane curvature, accumulation and transport of exopolycsccharides, photosynthesis and energy supplying (i.e. photosystem II stability and activity, cyclic electron transport, photorespiration, and C4 pathway), nuclear/chloroplastic gene expression regulation and protein processing, reactive oxygen species homeostasis, and salt signaling transduction. To our knowledge, this study of protein post-translational modifications regulation toward understanding the mechanism of algae palmella formation has not been reported before.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Dunaliella Salina

TISSUE(S): Whole Body

SUBMITTER: Shaojun Dai

LAB HEAD: Shaojun Dai

PROVIDER: PXD005501 | Pride | 2017-06-12

REPOSITORIES: Pride

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Publications

Salinity-Induced Palmella Formation Mechanism in Halotolerant Algae Dunaliella salina Revealed by Quantitative Proteomics and Phosphoproteomics.

Wei Sijia S Bian Yangyang Y Zhao Qi Q Chen Sixue S Mao Jiawei J Song Chunxia C Cheng Kai K Xiao Zhen Z Zhang Chuanfang C Ma Weimin W Zou Hanfa H Ye Mingliang M Dai Shaojun S

Frontiers in plant science 20170523

Palmella stage is critical for some unicellular algae to survive in extreme environments. The halotolerant algae Dunaliella salina is a good single-cell model for studying plant adaptation to high salinity. To investigate the molecular adaptation mechanism in salinity shock-induced palmella formation, we performed a comprehensive physiological, proteomics and phosphoproteomics study upon palmella formation of D. salina using dimethyl labeling and Ti4+-immobilized metal i ...[more]

PMID: 28588593

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Project description:Arabidopsis ecotypes of Sha and Ler showed differences in tolerance to salinity stress. A previous study indicated that a premature stop codon resulting in a truncated Response to ABA and Salt 1 (RAS1) protein in Sha contributes to the increased salt tolerance relative to Ler ecotype. Sha exhibited higher germination rates and longer roots on MS plate, presumably due to the decreased ABA sensitivity in Sha. More Sha plants also survived in soil after salt treatment with relatively lower electrolyte leakage when compared to Ler. Transcriptome analysis revealed that expression levels of many genes were changed between Sha and Ler ecotypes and by salt treatments. About 500 transcripts were commonly changed by at least one salinity effect and one ecotype effect, and 171 of them were co-regulated by all four comparisons. Transcripts involved in redox, secondary metabolism, auxin metabolism, photosynthesis, cell wall, and protein synthesis were mainly down-regulated by salinity effects, while transposable element genes, microRNA and antisense sequences, histone superfamily genes, and biotic stress related genes were significantly changed by Sha ecotype effects and only slightly by salinity. Several metabolic pathways such as stress, TCA, hormone/lipid/secondary metabolism, redox, development, and GO terms involved in stress, oxidation, and defense response were enriched by both salinity and ecotype effects. Ninety-five highly inducible genes were identified as candidates of RAS1 target genes and the functions involved hormone metabolism, biotic stress, RNA, DNA synthesis, protein metabolism, cell, and microRNA metabolism. All these results indicated that the Sha ecotype was possibly preconditioned to abiotic stress relative to Ler through regulation of signaling pathways and stress responsive gene expression. These comparative transcriptomic and analytical results also confirm the complexity of ABA responses and salt stress tolerance mechanisms, and they suggest additional targets for improving tolerance. Ten days old seedlings of two Arabidopsis ecotypes, Sha and Ler, were treated with 100 mM NaCl on MS plate. Plant materials were collected for RNA extraction at 4th days after treatments.

Dataset Information

Salinity-induced Palmella Formation Mechanism in Dunaliella salina Revealed by Phosphoproteomics

Publications

Salinity-Induced Palmella Formation Mechanism in Halotolerant Algae <i>Dunaliella salina</i> Revealed by Quantitative Proteomics and Phosphoproteomics.

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