In-depth and reproducibly quantitation of benchmark dataset consiting of 20 files
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: 
XIAOMENG SHEN
LAB HEAD: JUN QU
PROVIDER: PXD005590 | Pride | 2017-04-20
REPOSITORIES: Pride
Publications
An IonStar Experimental Strategy for MS1 Ion Current-Based Quantification Using Ultrahigh-Field Orbitrap: Reproducible, In-Depth, and Accurate Protein Measurement in Large Cohorts.
Shen Xiaomeng X Shen Shichen S Li Jun J Hu Qiang Q Nie Lei L Tu Chengjian C Wang Xue X Orsburn Benjamin B Wang Jianmin J Qu Jun J
Journal of proteome research 20170525 7
In-depth and reproducible protein measurement in many biological samples is often critical for pharmaceutical/biomedical proteomics but remains challenging. MS1-based quantification using quadrupole/ultrahigh-field Orbitrap (Q/UHF-Orbitrap) holds great promise, but the critically important experimental approaches enabling reliable large-cohort analysis have long been overlooked. Here we described an IonStar experimental strategy achieving excellent quantitative quality of MS1 quantification. Key ...[more]
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