Proteomics

Dataset Information

54

Relating human gut metagenome and metaproteome


ABSTRACT: Gut microbiome research is rapidly moving towards the functional characterization of the microbiota by means of shotgun meta-omics. Here, we selected a cohort of healthy subjects from an indigenous and monitored Sardinian population to analyze their gut microbiota using both shotgun metagenomics and shotgun metaproteomics. We found a considerable divergence between genetic potential and functional activity of the human healthy gut microbiota, in spite of a quite comparable taxonomic structure revealed by the two approaches. Investigation of inter-individual variability of taxonomic features revealed Bacteroides and Akkermansia as remarkably conserved and variable in abundance within the population, respectively. Firmicutes-driven butyrogenesis (mainly due to Faecalibacterium spp.) was shown to be the functional activity with the higher expression rate and the lower inter-individual variability in the study cohort, highlighting the key importance of the biosynthesis of this microbial by-product for the gut homeostasis. The taxon-specific contribution to functional activities and metabolic tasks was also examined, giving insights into the peculiar role of several gut microbiota members in carbohydrate metabolism (including polysaccharide degradation, glycan transport, glycolysis and short-chain fatty acid production). In conclusion, our results provide useful indications regarding the main functions actively exerted by the gut microbiota members of a healthy human cohort, and support metaproteomics as a valuable approach to investigate the functional role of the gut microbiota in health and disease.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): human gut metagenome  

TISSUE(S): Feces

DISEASE(S): Not Available

SUBMITTER: Alessandro Tanca  

LAB HEAD: Sergio Uzzau

PROVIDER: PXD005780 | Pride | 2017-07-19

REPOSITORIES: Pride

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Publications

Potential and active functions in the gut microbiota of a healthy human cohort.

Tanca Alessandro A   Abbondio Marcello M   Palomba Antonio A   Fraumene Cristina C   Manghina Valeria V   Cucca Francesco F   Fiorillo Edoardo E   Uzzau Sergio S  

Microbiome 20170714 1


The study of the gut microbiota (GM) is rapidly moving towards its functional characterization by means of shotgun meta-omics. In this context, there is still no consensus on which microbial functions are consistently and constitutively expressed in the human gut in physiological conditions. Here, we selected a cohort of 15 healthy subjects from a native and highly monitored Sardinian population and analyzed their GMs using shotgun metaproteomics, with the aim of investigating GM functions actua  ...[more]

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