Proteomics

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Mouse caecum vs faeces - Metaproteogenomics Reveals Taxonomic and Functional Changes between Cecal and Fecal Microbiota in Mouse


ABSTRACT: We compared the microbiota of paired mouse caecal contents and faeces by applying a multi-omic approach, including 16S rDNA sequencing, shotgun metagenomics, and shotgun metaproteomics. The aim of the study was to verify whether faecal samples are a reliable proxy for the mouse colonic luminal microbiota, as well as to identify changes in taxonomy and functional activity between caecal and faecal microbial communities, which have to be carefully considered when using stool as sample for mouse gut microbiota investigations.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): mouse gut metagenome  

TISSUE(S): Intestine

DISEASE(S): Not Available

SUBMITTER: Alessandro Tanca  

LAB HEAD: Sergio Uzzau

PROVIDER: PXD004911 | Pride | 2017-03-31

REPOSITORIES: pride

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Publications

Metaproteogenomics Reveals Taxonomic and Functional Changes between Cecal and Fecal Microbiota in Mouse.

Tanca Alessandro A   Manghina Valeria V   Fraumene Cristina C   Palomba Antonio A   Abbondio Marcello M   Deligios Massimo M   Silverman Michael M   Uzzau Sergio S  

Frontiers in microbiology 20170314


Previous studies on mouse models report that cecal and fecal microbial communities may differ in the taxonomic structure, but little is known about their respective functional activities. Here, we employed a metaproteogenomic approach, including 16S rRNA gene sequencing, shotgun metagenomics and shotgun metaproteomics, to analyze the microbiota of paired mouse cecal contents (CCs) and feces, with the aim of identifying changes in taxon-specific functions. As a result, Gram-positive anaerobes wer  ...[more]

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