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Proteomic changes in HepaRG cells exposed to mycotoxins

ABSTRACT: Proteomic changes associated with the individual and combined exposures of deoxynivalenol (DON) and zearalenone (ZEA) were investigated in human hepatocytes (HepaRG cell line) after only 1h exposure and at low cytotoxicity levels using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Marie-Caroline Smith

LAB HEAD: Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, Plouzané

PROVIDER: PXD005840 | Pride | 2017-12-13

REPOSITORIES: Pride

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Differential impacts of individual and combined exposures of deoxynivalenol and zearalenone on the HepaRG human hepatic cell proteome.

Smith Marie-Caroline MC Timmins-Schiffman Emma E Coton Monika M Coton Emmanuel E Hymery Nolwenn N Nunn Brook L BL Madec Stéphanie S

Journal of proteomics 20171205

Numerous surveys have highlighted the natural co-occurrence of deoxynivalenol (DON) and zearalenone (ZEA) mycotoxins in food and feed. Nevertheless, data regarding cellular mechanisms involved in response to their individual and simultaneous exposures are lacking. In this study, in order to analyze how low mycotoxin doses could impact cellular physiology and homeostasis, proteomic profiles of proliferating human hepatic cells (HepaRG) exposed for 1h and 24h to low DON and ZEA cytotoxicity levels ...[more]

PMID: 29208510

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Proteomic changes in HepaRG cells exposed to mycotoxins, part 2

Project description:Proteomic changes associated with the individual and combined exposures of deoxynivalenol (DON) and zearalenone (ZEA) were investigated in human hepatocytes (HepaRG cell line) after 24 hour expousre and at low cytotoxicity levels using liquid chromatography coupled to tandem mass spectrometry.

2017-12-13 | PXD006267 | Pride

Omics-characterization of tebufenpyrad effects on HepaRG : cell culture medium

Project description:The biological effects of the pesticide and complex I inhibitor tebufenpyrad (TEBU) on liver cells were investigated by proteomic approaches. Cell culture media were analyzed in dose-response experiments on differentiated HepaRG cell line. Moreover, kinetics were also investigated on HepaRG cells.

2022-10-06 | PXD031045 | Pride

Expression data from LCM-captured Zea mays root cells after challenge with Meloidogyne incognita

Project description:The differentiation of specialized feeding sites in Zea mays root cells in response to nematode infestation involves substantial cellular reprogramming of host cells that is not well characterized at the molecular level. Expression data was generated from Zea mays root cells undergoing giant cell formation due to nematode infestation and from non-infested control root cells. Cells were laser captured 14 and 21 days after infestation. Each time point (14 day and 21 day) consisted of three biological replicates per treatment (control root cells or giant cells). Control cells were captured from an area ~13,000,000 um2 in size and giant cells were captured from an area ~5,000,000 um2 in size. RNA samples were isolated using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, USA). RNA amplifications were carried out with the NuGEN WT-Ovation Pico kit.

2012-04-27 | E-GEOD-37556 | biostudies-arrayexpress

Omics-characterization of tebufenpyrad effects on liver cells

Project description:The biological effects of the pesticide and complex I inhibitor tebufenpyrad (TEBU) on liver cells were investigated by proteomic approaches. Cellular contents were analyzed in dose-response experiments on differentiated HepaRG cell line. Moreover, kinetics were also investigated on HepaRG cells.

2022-10-06 | PXD030822 | Pride

Subcellular proteomics for determining iron limited remodeling of plastids in the model diatom Thalassiosira pseudonana

Project description:Diatoms are important primary producers in the world’s oceans, yet their growth is constrained in large regions by low bioavailable iron (Fe). Low Fe-induced limitation of primary production is due to requirements for Fe in components of essential metabolic pathways including photosynthesis and other chloroplast plastid functions. Studies have shown that under Fe-limited stress, diatoms alter plastid-specific processes, including components of electron transport. These physiological changes suggest changes of protein content and their abundance within the diatom plastid. While in-silico predictions provide putative information on plastid-localized proteins, knowledge of diatom plastid proteins remains limited in comparison to model photosynthetic organisms. To characterize proteins enriched in diatom plastids we have used shotgun proteomics to assess the proteome of subcellular plastid-enriched fractions from Thalassiosira pseudonana. To improve our understanding of how the plastid proteome is remodeled in response to Fe limitation, proteome sequencing has been performed on T. pseudonana grown under Fe replete and limited conditions. These analyses have shown that Fe limitation regulates major metabolic pathways in the plastid, including the Calvin cycle, as well as changes in light harvesting protein expression. In-silico localization predictions of proteins identified in this plastid-enriched proteome allowed for an in-depth comparison of theoretical vs observed plastid-localization, providing evidence for the potential of additional protein import pathways into the diatom plastid.

2023-08-04 | PXD040824 | Pride

Genome-wide midrange transcription profiles reveal expression level relationships in human tissue specification - GeneNote

Project description:PolyA+ RNA samples from the twelve healthy human tissues were purchased from Clontech (Palo Alto, CA). Each RNA sample is typically composed of a pool of 10-25 individuals. The expression intensity of mRNA was assayed across five microarrays (Affymetrix GeneChips U95A–E). Please note GeneNote is now retired: https://genecards.weizmann.ac.il/cgi-bin/genenote/home_page.pl. This dataset is part of the TransQST collection.

2003-11-19 | E-GEOD-803 | biostudies-arrayexpress

Barley host response to the direct application of the trichothecene mycotoxin deoxynivalenol

Project description:Barley florets (cv. Morex) were treated with 2.0 microgram deoxynivalenol per floret via a 10 microliter solution or mock inoculated with water. Samples were collected at 1, 12, 24, and 48 hours after inoculation. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Stephanie A. Gardiner. The equivalent experiment is BB62 at PLEXdb.] time: 1 hai - treated or untreated: Deoxynivalenol(3-replications); time: 1 hai - treated or untreated: Water(3-replications); time: 12 hai - treated or untreated: Deoxynivalenol(3-replications); time: 12 hai - treated or untreated: Water(3-replications); time: 24 hai - treated or untreated: Deoxynivalenol(3-replications); time: 24 hai - treated or untreated: Water(3-replications); time: 48 hai - treated or untreated: Deoxynivalenol(3-replications); time: 48 hai - treated or untreated: Water(3-replications)

2011-11-22 | E-GEOD-33405 | biostudies-arrayexpress

Phosphoprotein network analysis of white adipose tissues unveils deregulated pathways in response to high-fat diet

Project description:Despite wide efforts in the last decade, signaling aberrations associated with obesity remain enigmatic. Here, we carried out phosphoproteomic analysis of mouse white adipose tissues (WAT) upon low-fat diet (LFD) and high-fat diet (HFD) to dissect underlying molecular mechanisms of obesity. Of the 7696 phosphopeptides quantified, 191 proteins including various insulin-responsive proteins and metabolic enzymes functioning in lipid homeostasis, exhibited differential phosphorylation with high-fat feeding. Kinase predictions and integrated network analysis identified several deregulated kinase signaling pathways, and suggested possibilities of HFD-induced transcriptional rewiring. Further, functional validation of a novel HFD-responsive site on cytoplasmic acetyl-coA forming ACSS2 (S263) suggested that the phosphorylation is important in regulating insulin signaling and maintaining triglyceride levels. This study represents one of the first comprehensive phosphoproteome data in mouse obesity models, and describes a systems-level approach for identifying deregulated molecular events and potential therapeutic targets in the context of high-fat feeding and adipocyte perturbation.

2016-05-20 | PXD002927 | Pride

Multi-stress experiment on Pseudonitzschia multiseries

Project description:A changing climate is altering many ocean properties that consequently will modify marine productivity. Previous phytoplankton manipulation studies have focused on individual or subsets of these properties. Here, we investigate the cumulative effects of multi-faceted change on a subantarctic diatom Pseudonitzschia multiseries by concurrently manipulating five stressors (light/nutrients/CO2/temperature/iron) that primarily control its physiology, and explore underlying reasons for altered physiological performance. Climate change enhances diatom growth mainly owing to warming and iron enrichment, and both properties decrease cellular nutrient quotas, partially offsetting any effects of decreased nutrient supply by 2100. Physiological diagnostics and comparative proteomics demonstrate the joint importance of individual and interactive effects of temperature and iron, and reveal biased future predictions from experimental outcomes when only a subset of multi-stressors is considered. Our findings for subantarctic waters illustrate how composite regional studies are needed to provide accurate global projections of future shifts in productivity and distinguish underlying species-specific physiological mechanisms.

2017-05-19 | PXD006468 | Pride

Loss of ZEB2 in basal breast cancer cells

Project description:Study of loss of ZEB2 in breast cancer cells in vitro and in vivo: gene expression and phenotypic switch to more benign behavior of the cancer cells. Morphological, functional and gene microarray analysis following ZEB2 knockdown in MDAMB231 cells reveals gain of epithelial differentiation, reduction of migration, invasion and metastatic capability. The measurement of ZEB2 transcriptional activity in tumor cells efficiently predicts the probability of breast cancer patient survival. Global gene expression profiles were measured using Affymetrix U133 plus2 microarrays in MDAMB231 cells (control and ZEB2 shRNA and siRNA knockdown cells on plastic)

2016-03-15 | E-GEOD-27966 | biostudies-arrayexpress

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