Quantitative proteomic analyses of budding yeast cells harboring an additional chromosome
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: 
Amanda Del Rosario
LAB HEAD: Amanda M Del Rosario
PROVIDER: PXD006009 | Pride | 2017-04-10
REPOSITORIES: Pride
Publications
Aneuploidy Causes Non-genetic Individuality.
Beach Rebecca R RR Ricci-Tam Chiara C Brennan Christopher M CM Moomau Christine A CA Hsu Pei-Hsin PH Hua Bo B Silberman Rebecca E RE Springer Michael M Amon Angelika A
Cell 20170401 2
Phenotypic variability is a hallmark of diseases involving chromosome gains and losses, such as Down syndrome and cancer. Allelic variances have been thought to be the sole cause of this heterogeneity. Here, we systematically examine the consequences of gaining and losing single or multiple chromosomes to show that the aneuploid state causes non-genetic phenotypic variability. Yeast cell populations harboring the same defined aneuploidy exhibit heterogeneity in cell-cycle progression and respons ...[more]
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Project description:Aneuploidy causes severe developmental defects and is a near universal feature of tumor cells. Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. We show that although protein levels largely scale with gene copy number, subunits of multi-protein complexes are notable exceptions. Posttranslational mechanisms attenuate their expression when their encoding genes are in excess. Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis. Indeed aneuploid cells harbor increased levels of reactive oxygen species (ROS). Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains. Our results show that aneuploidy causes alterations in metabolism and redox homeostasis. Cells respond to these alterations through both transcriptional and posttranscriptional mechanisms.
2014-08-14 | PXD001019 | Pride
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2020-06-16 | GSE146791 | GEO
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2013-09-24 | E-GEOD-49247 | biostudies-arrayexpress
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2022-09-19 | MTBLS1612 | MetaboLights
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2023-09-15 | PXD042785 | Pride
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2021-03-11 | GSE168674 | GEO