Proteomics

Dataset Information

32

MoDCs HPLC-MS/MS


ABSTRACT: To investigate the allergenicity of the major birch pollen allergen Bet v 1 and the impact of known adjuvants coming from pollen, such as lipopolysaccharide (LPS), we performed quantitative proteome analysis of stimulated monocyte-derived dendritic cells (moDCs). Thus, we treated cells with birch pollen extract (BPE), a recombinant variant of Bet v 1 or LPS followed by proteomic profiling by means of high performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) using isobaric labelling.

INSTRUMENT(S): Q Exactive Plus, Q Exactive

ORGANISM(S): Homo sapiens  

TISSUE(S): Blood

DISEASE(S): Allergy,Pollen Allergy

SUBMITTER: Lisa Strasser  

LAB HEAD: Christian G. Huber

PROVIDER: PXD006196 | Pride | 2017-12-01

REPOSITORIES: Pride

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Publications

Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells.

Strasser L L   Dang H-H HH   Schwarz H H   Asam C C   Ferreira F F   Horejs-Hoeck J J   Huber C G CG  

Scientific reports 20171130 1


Worldwide, more than 1 billion people suffer from allergic diseases. However, until now it is not fully understood how certain proteins can induce allergic immune responses, while others cannot. Studies suggest that allergenicity is a process not only determined by properties of the allergen itself but also by costimulatory factors, that are not classically associated with allergic reactions. To investigate the allergenicity of the major birch pollen allergen Bet v 1 and the impact of adjuvants  ...[more]

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