Proteomics

Dataset Information

46

Tropheryma whipplei proteome - Analyses of new genomic, transcriptomic or proteomic data commonly result in trashing many unidentified data escaping the ‘canonical’ DNA-RNA-protein scheme


ABSTRACT: Analyses of new genomic, transcriptomic or proteomic data commonly result in trashing many unidentified data escaping the ‘canonical’ DNA-RNA-protein scheme. Testing systematic exchanges of nucleotides over long stretches produces inversed RNA pieces (here named “swinger” RNA) differing from their template DNA. These may explain some trashed data. Here analyses of genomic, transcriptomic and proteomic data of the pathogenic Tropheryma whipplei according to canonical genomic, transcriptomic and translational 'rules' resulted in trashing 58.9% of DNA, 37.7% RNA and about 85% of mass spectra (corresponding to peptides). In the trash, we found numerous DNA/RNA fragments compatible with “swinger” polymerization. Genomic sequences covered by «swinger» DNA and RNA are 3X more frequent than expected by chance and explained 12.4 and 20.8% of the rejected DNA and RNA sequences, respectively. As for peptides, several match with “swinger” RNAs, including some chimera, translated from both regular, and «swinger» transcripts, notably for ribosomal RNAs. Congruence of DNA, RNA and peptides resulting from the same swinging process suggest that systematic nucleotide exchanges increase coding potential, and may add to evolutionary diversification of bacterial populations.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Tropheryma whipplei TW08/27  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Herve Seligmann  

LAB HEAD: Herve Seligmann

PROVIDER: PXD006238 | Pride | 2017-12-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AC-GT01b.msf Msf
AC-GT02a.msf Msf
AC-GT03_b.msf Msf
AC-GT04b.msf Msf
AC-GT05b.msf Msf
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