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HIV-Tat treatment of human neurons in vitro over time: towards a model for the molecular pathology of HIV-associated neurocognitive disorders (~90% cell culture confluence dataset)

ABSTRACT: We have used LC-MSMS discovery proteomics to identify the quantitative, cell-wide changes that occur when non-transformed, differentiated human neurons are treated with HIV-Tat over time. This may represent a novel cell culture model for initial HIV-associated neurocognitive disorder progression.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neuron, Cell Culture, Neural Stem Cell, Embryonic Stem Cell

DISEASE(S): Human Immunodeficiency Virus Infectious Disease

SUBMITTER: Kim Gurwitz

LAB HEAD: Prof. Jonathan Blackburn

PROVIDER: PXD006314 | Pride | 2017-05-19

REPOSITORIES: Pride

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Time-Dependent, HIV-Tat-Induced Perturbation of Human Neurons <i>In Vitro</i>: Towards a Model for the Molecular Pathology of HIV-Associated Neurocognitive Disorders.

Gurwitz Kim T KT Burman Richard J RJ Murugan Brandon D BD Garnett Shaun S Ganief Tariq T Soares Nelson C NC Raimondo Joseph V JV Blackburn Jonathan M JM

Frontiers in molecular neuroscience 20170529

A significant proportion of human immunodeficiency virus type 1 (HIV)-positive individuals are affected by the cognitive, motor and behavioral dysfunction that characterizes HIV-associated neurocognitive disorders (HAND). While the molecular etiology of HAND remains largely uncharacterized, HIV transactivator of transcription (HIV-Tat) is thought to be an important etiological cause. Here we have used mass spectrometry (MS)-based discovery proteomics to identify the quantitative, cell-wide chang ...[more]

PMID: 28611588

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