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TAILS N-terminomics of the human dental pulp stroma and the odontoblast layer

ABSTRACT: We report the targeted analysis of the human dental pulp stroma and the odontoblast layer using the positional proteomics technique TAILS N-terminomics, which allowed us to capture both naturally blocked and unblocked protein N-termini, and to identify differences between e.g. the dental pulp proteomes of older and younger donors. Noteworthy, these differences were most pronounced in the odontoblast region. Note: This study is a follow-up on PXD002264.

INSTRUMENT(S): 6520 Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Dental Pulp

SUBMITTER: Ulrich ECKHARD

LAB HEAD: Prof. Christopher Mark Overall

PROVIDER: PXD006557 | Pride | 2017-10-20

REPOSITORIES: Pride

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The Human Odontoblast Cell Layer and Dental Pulp Proteomes and N-Terminomes.

Abbey S R SR Eckhard U U Solis N N Marino G G Matthew I I Overall C M CM

Journal of dental research 20171016 3

The proteome and N-terminome of the human odontoblast cell layer were identified for the first time by shotgun proteomic and terminal amine isotopic labeling of substrates (TAILS) N-terminomic analyses, respectively, and compared with that of human dental pulp stroma from 26 third molar teeth. After reverse-phase liquid chromatography-tandem mass spectrometry, >170,000 spectra from the shotgun and TAILS analyses were matched by 4 search engines to 4,888 and 12,063 peptides in the odontoblast cel ...[more]

PMID: 29035686

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