Human Dental Pulp TAILS N-terminomics

ABSTRACT: We report the targeted analysis of the human dental pulp proteome and N-terminome using the positional proteomics technique TAILS, which allowed us to capture both naturally blocked and unblocked protein N-termini. Furthermore, by using a proteomically barely studied human tissue in combination with a non-shotgun proteomics approach, we were able to identify many so far missing proteins (PE level 2-4), and established together with our previously published studies on human erythrocytes (Lange et al. 2014) and platelets (Prudova et al. 2014), a general workflow suitable for the in-depth and high-throughput analysis of protein N-termini in human specimen, as envisioned by the Chromosome-centric Human Proteome Project.

REANALYSED by: PAe005343

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Dental Pulp

SUBMITTER: Ulrich ECKHARD  

LAB HEAD: Prof. Christopher Mark Overall

PROVIDER: PXD002264 | Pride | 2015-08-18

REPOSITORIES: Pride