Proteomics

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Human Dental Pulp TAILS N-terminomics

ABSTRACT: We report the targeted analysis of the human dental pulp proteome and N-terminome using the positional proteomics technique TAILS, which allowed us to capture both naturally blocked and unblocked protein N-termini. Furthermore, by using a proteomically barely studied human tissue in combination with a non-shotgun proteomics approach, we were able to identify many so far missing proteins (PE level 2-4), and established together with our previously published studies on human erythrocytes (Lange et al. 2014) and platelets (Prudova et al. 2014), a general workflow suitable for the in-depth and high-throughput analysis of protein N-termini in human specimen, as envisioned by the Chromosome-centric Human Proteome Project.

REANALYSED by: PAe005343

INSTRUMENT(S): 6520 Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Dental Pulp

SUBMITTER: Ulrich ECKHARD  

LAB HEAD: Prof. Christopher Mark Overall

PROVIDER: PXD002264 | Pride | 2015-08-18

REPOSITORIES: Pride

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Publications

The Human Dental Pulp Proteome and N-Terminome: Levering the Unexplored Potential of Semitryptic Peptides Enriched by TAILS to Identify Missing Proteins in the Human Proteome Project in Underexplored Tissues.

Eckhard Ulrich U   Marino Giada G   Abbey Simon R SR   Tharmarajah Grace G   Matthew Ian I   Overall Christopher M CM  

Journal of proteome research 20150814 9

An underexplored yet widespread feature of the human proteome is the proteolytic proteoforms of proteins. We used terminal amine isotopic labeling of substrates (TAILS), a high-content N-terminal positional proteomics technique, for in-depth characterization of the human dental pulp proteome from its N-terminome and to provide data for the Chromosome-centric Human Proteome Project (C-HPP). Dental pulp is a unique connective tissue maintaining tooth sensation and structure by supporting a single  ...[more]

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