Proteomics

Dataset Information

0

Ubiquitome analysis of UHRF1 and UHRF2-deficient mESCs

ABSTRACT: The E3 ligase UHRF1 is an essential epigenetic cofactor for DNMT1 dependent maintenance DNA methylation, which provides a binding platform for DNMT1 by both cooperative binding of histones and hemi-methylated DNA as well as by ubiquitinating histone H3. Here, we conduct a comprehensive screen to identify novel ubiquitination targets of UHRF1 and its paralogue UHRF2 by comparing the ubiquitome of wildtype (wt), UHRF1- and UHRF2-deficient mouse embryonic stem cells. With an antibody-dependent enrichment of ubiquitin remnant motif-containing peptides followed by isobaric-labeling based quantitative mass spectrometry, we find both known and novel E3 ligase substrates of UHRF1 involved in a variety of biological processes such as RNA processing, DNA methylation and DNA damage repair.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: Elisabeth Karg  

LAB HEAD: Heinrich Leonhardt

PROVIDER: PXD006593 | Pride | 2017-10-24

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Ubiquitome Analysis Reveals PCNA-Associated Factor 15 (PAF15) as a Specific Ubiquitination Target of UHRF1 in Embryonic Stem Cells.

Karg Elisabeth E   Smets Martha M   Ryan Joel J   Forné Ignasi I   Qin Weihua W   Mulholland Christopher B CB   Kalideris Georgia G   Imhof Axel A   Bultmann Sebastian S   Leonhardt Heinrich H  

Journal of molecular biology 20171018 24

Ubiquitination is a multifunctional posttranslational modification controlling the activity, subcellular localization and stability of proteins. The E3 ubiquitin ligase ubiquitin-like PHD and RING finger domain-containing protein 1 (UHRF1) is an essential epigenetic factor that recognizes repressive histone marks as well as hemi-methylated DNA and recruits DNA methyltransferase 1. To explore enzymatic functions of UHRF1 beyond epigenetic regulation, we conducted a comprehensive screen in mouse e  ...[more]

Similar Datasets

Proteomics Non - canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cell
2024-02-12 | PXD043254 | Pride
Proteomics Critical role of the UBL-domain in stimulating the E3 ubiquitin ligase activity of UHRF1 towards chromatin
2018-11-04 | PXD011510 | Pride
Proteomics Hemi-methylated DNA regulates DNA methylation inheritance through allosteric control of H3 ubiquitylation by UHRF1
2016-08-25 | PXD003983 | Pride
Proteomics Identificaiton of novel acetylation sites in UHRF1 mediated by acetyltransferase MOF
2024-03-06 | PXD040426 | Pride
Proteomics DNMT1 IP-MS alalysis to identify ubiquitin-dependent interacting partners
2020-02-25 | PXD015282 | Pride
Transcriptomics Gene expression profiles of uhrf1 mutant zebrafish
2015-02-09 | E-GEOD-55339 | biostudies-arrayexpress
Unknown Effect of MOF mediated UHRF1 acetylation on global DNA methyaltion maintenance in mouse embryonic stem cells.
2024-03-05 | GSE225947 | GEO
Unknown LSH facilitates DNA methylation primarily by promoting UHRF1 DNA accessibility and DNA methylation by DNMT1
2020-12-01 | GSE136931 | GEO
Unknown Critical role of the UBL-domain in stimulating the E3 ubiquitin ligase activity of UHRF1 towards chromatin
2018-08-29 | GSE119120 | GEO
Unknown Gene expression profiles of uhrf1 mutant zebrafish
2015-02-09 | GSE55339 | GEO