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Quantitative proteomic analysis of oocytes from high-fat diet (HFD)-fed and normal diet mice

ABSTRACT: In order to establish an obese mouse model, female mice were continuously fed with a high-fat diet (HFD) or a normal diet (control) for 16 weeks beginning at three weeks of age. In this paper, these mice are termed ‘HFD mice’ and ‘control mice’, respectively. Accordingly, we call their oocytes ‘HFD oocytes’ and ‘control oocytes’. Substantial evidence indicates that the effects of maternal obesity on embryo/offspring development can be attributed to factors within the oocyte (9). To identify such potential effectors, we performed a comparative proteomic analysis of ovulated MII oocytes from control and HFD mice.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Oocyte

SUBMITTER: hao zhang

LAB HEAD: Guo, Xuejiang

PROVIDER: PXD006641 | Pride | 2018-05-24

REPOSITORIES: Pride

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Loss of TIGAR Induces Oxidative Stress and Meiotic Defects in Oocytes from Obese Mice.

Wang Haichao H Cheng Qing Q Li Xiaoyan X Hu Feifei F Han Longsen L Zhang Hao H Li Ling L Ge Juan J Ying Xiaoyan X Guo Xuejiang X Wang Qiang Q

Molecular & cellular proteomics : MCP 20180518 7

Maternal obesity has been reported to impair oocyte quality in mice, however, the underlying mechanisms remain unclear. In the present study, by conducting a comparative proteomic analysis, we identified a reduced expression of TIGAR (TP53-induced glycolysis and apoptosis regulator) protein in ovulated oocytes from high-fat diet (HFD)-fed mice. Specific depletion of TIGAR in mouse oocytes results in the marked elevation of reactive oxygen species (ROS) levels and the failure of meiotic apparatus ...[more]

PMID: 29776966

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