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Bladder Tumours O-Glycoproteomics

ABSTRACT: Bladder carcinogenesis and tumour progression is accompanied by profound alterations in protein glycosylation. These include the presence of short chain O-glycans such as Tn and T antigens and their sialylated counterparts sialyl-Tn(STn) and sialyl-T(ST). The presence of such structures is commonly associated with poor prognosis. In search for prognosis biomarkers and novel therapeutic targets we have screened, using immunohistochemistry, a series of bladder tumours with differing clinicopathology for these short-chain O-glycans. Aiming at identifying protein targets, we have studied the O-glycoproteome of a pool of STn positive tumours from muscle invasive bladder cancer (MIBC) patients.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bladder

DISEASE(S): Urinary Bladder Cancer

SUBMITTER: André Silva

LAB HEAD: José Alexandre Ferreira

PROVIDER: PXD006813 | Pride | 2017-08-17

REPOSITORIES: Pride

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In silico approaches for unveiling novel glycobiomarkers in cancer.

Azevedo Rita R Silva André M N AMN Reis Celso A CA Santos Lúcio Lara LL Ferreira José Alexandre JA

Journal of proteomics 20170804

Glycosylation is one of the most common and dynamic post-translational modification of cell surface and secreted proteins. Cancer cells display unique glycosylation patterns that decisively contribute to drive oncogenic behavior, including disease progression and dissemination. Moreover, alterations in glycosylation are often responsible for the creation of protein signatures holding significant biomarker value and potential for targeted therapeutics. Accordingly, many analytical protocols have ...[more]

PMID: 28782717

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