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SWATH-MS analysis of transgenic tobacco overexpressing 3-hydroxy-3-methylglutaryl-CoA synthase

ABSTRACT: 3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) is the second enzyme in the mevalonate pathway. While the recombinant Brassica juncea HMGS1 (BjHMGS1) mutant S359A displayed 10-fold higher enzyme activity than wild-type (wt) BjHMGS1, transgenic tobacco overexpressing S359A (OE-S359A) exhibited greater sterol content, growth rate and seed yield than OE-wtBjHMGS1. To explore the mechanism of HMGS and its mutant (S359A) in promotion of plant growth, SWATH-MS quantitative proteomics analysis was performed.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Nicotiana Tabacum (common Tobacco)

TISSUE(S): Seedling

SUBMITTER: Pan Liao

LAB HEAD: Mee-Len Chye

PROVIDER: PXD006869 | Pride | 2021-01-04

REPOSITORIES: Pride

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Overexpression and Inhibition of 3-Hydroxy-3-Methylglutaryl-CoA Synthase Affect Central Metabolic Pathways in Tobacco.

Liao Pan P Lung Shiu-Cheung SC Chan Wai Lung WL Hu Menglong M Kong Geoffrey Kwai-Wai GK Bach Thomas J TJ Hao Quan Q Lo Clive C Chye Mee-Len ML

Plant & cell physiology 20210301 1

Little has been established on the relationship between the mevalonate (MVA) pathway and other metabolic pathways except for the sterol and glucosinolate biosynthesis pathways. In the MVA pathway, 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) catalyzes the condensation of acetoacetyl-CoA and acetyl-CoA to form 3-hydroxy-3-methylglutaryl-coenzyme A. Our previous studies had shown that, while the recombinant Brassica juncea HMGS1 (BjHMGS1) mutant S359A displayed 10-fold higher enzyme activity tha ...[more]

PMID: 33340324

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