Proteomics

Dataset Information

145

ZBTB2 as a novel reader for unmethylated DNA


ABSTRACT: In this study, we investigated the dynamics during differentiation of the in vivo binding sites of ZBTB2, a putative reader for unmethylated DNA. We performed DNA pull-downs followed by mass spectrometry, using a genomic sequence containing either unmethylated or methylated CpGs, to study the influence of DNA methylation on ZBTB2 binding. Additionally, we performed interaction proteomics to identify ZBTB2 interaction partners. We found that ZBTB2 recruits a zinc finger module of three proteins to unmethylated DNA.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Mus musculus  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Ino Karemaker  

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD007099 | Pride | 2018-01-29

REPOSITORIES: Pride

altmetric image

Publications

ZBTB2 reads unmethylated CpG island promoters and regulates embryonic stem cell differentiation.

Karemaker Ino D ID   Vermeulen Michiel M  

EMBO reports 20180201 4


Proteins that bind to DNA depending on its methylation status play an important role in methylation-mediated regulation of gene expression. Using a variety of genomics and proteomics approaches, we identify zinc finger and BTB domain-containing protein 2 (ZBTB2) as a reader of unmethylated DNA in mouse embryonic stem cells. ZBTB2 preferentially binds to CpG island promoters, where it acts as a transcriptional activator. The binding of ZBTB2 to its targets is direct and independent of two other z  ...[more]

Similar Datasets

2018-10-11 | PXD010512 | Pride
2019-01-17 | PXD009513 | Pride
2018-01-29 | PXD006575 | Pride
2017-09-19 | PXD003429 | Pride
2018-10-24 | PXD006365 | Pride
2014-01-01 | E-GEOD-47790 | ArrayExpress
2018-10-15 | PXD009745 | Pride
2018-11-21 | PXD010162 | Pride
| PRJNA395548 | ENA
2019-06-27 | PXD011767 | Pride