Proteomics

Dataset Information

34

NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells


ABSTRACT: The Nucleosome Remodeling and Deacetylase (NuRD) complex plays an important role in gene expression regulation, stem cell self-renewal, and lineage commitment. Yet little is known about the dynamics of NuRD during cellular differentiation. Here, we study these dynamics using genome-wide profiling and quantitative interaction proteomics in mouse embryonic stem cells (ESCs) and neural progenitor cells (NPCs). The genomic targets of NuRD are highly dynamic during differentiation, with most binding occurring at cell-type specific promoters and enhancers. We identify ZFP296 as a novel, ESC-specific NuRD interactor that also interacts with the SIN3A complex. ChIP-sequencing in Zfp296 knockout (KO) ESCs reveals decreased NuRD binding both genome-wide and at ZFP296 binding sites, although this has little effect on the transcriptome. Nevertheless, Zfp296 KO ESCs exhibit delayed induction of lineage-specific markers upon differentiation to embryoid bodies. In summary, we identify an ESC-specific NuRD interacting protein which regulates genome-wide NuRD binding and cellular differentiation.

INSTRUMENT(S): LTQ Orbitrap Velos, Orbitrap Fusion, Q Exactive

ORGANISM(S): Mus musculus  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Ino Karemaker  

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD010512 | Pride | 2018-10-11

REPOSITORIES: Pride

altmetric image

Publications

NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells.

Kloet Susan L SL   Karemaker Ino D ID   van Voorthuijsen Lisa L   Lindeboom Rik G H RGH   Baltissen Marijke P MP   Edupuganti Raghu R RR   Poramba-Liyanage Deepani W DW   Jansen Pascal W T C PWTC   Vermeulen Michiel M  

Nature communications 20181102 1


The nucleosome remodeling and deacetylase (NuRD) complex plays an important role in gene expression regulation, stem cell self-renewal, and lineage commitment. However, little is known about the dynamics of NuRD during cellular differentiation. Here, we study these dynamics using genome-wide profiling and quantitative interaction proteomics in mouse embryonic stem cells (ESCs) and neural progenitor cells (NPCs). We find that the genomic targets of NuRD are highly dynamic during differentiation,  ...[more]

Similar Datasets

2016-05-19 | PXD003758 | Pride
| GSE80708 | GEO
2019-03-26 | PXD009855 | Pride
2016-08-02 | PXD003614 | Pride
2016-07-28 | E-GEOD-84905 | ArrayExpress
2019-07-04 | PXD011782 | Pride
2016-10-10 | PXD003856 | Pride
2012-01-03 | E-GEOD-27714 | ArrayExpress
2015-09-03 | E-GEOD-16364 | ExpressionAtlas
2012-01-03 | E-GEOD-27841 | ArrayExpress