AP-MS analysis of IFIT1 and 5 interactions in interferon-stimulated human cells

ABSTRACT: This project sought to identify interactions of InterFeron Induced Tetratricopeptide repeat (IFIT) proteins 1 ad 5 in human cells. SILAC-labelled human HEK293T cells were transfected with FLAG-tagged human IFIT1 and IFIT5 pulldowns. After transfection, the cells were treated with human interferon 2-alpha and FLAG-immunoprecipitations performed to identify IFIT-binding proteins. Cells were then subject to LS/MS-MS analysis on a Orbitrap Fusion and data analysed in Maxquant v1.5.7.4

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293et Cell

SUBMITTER: Edward Emmott  

LAB HEAD: Trevor Sweeney

PROVIDER: PXD007584 | Pride | 2018-03-16

REPOSITORIES: Pride