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AP-MS analysis of IFIT1 and 5 interactions in interferon-stimulated human cells

ABSTRACT: This project sought to identify interactions of InterFeron Induced Tetratricopeptide repeat (IFIT) proteins 1 ad 5 in human cells. SILAC-labelled human HEK293T cells were transfected with FLAG-tagged human IFIT1 and IFIT5 pulldowns. After transfection, the cells were treated with human interferon 2-alpha and FLAG-immunoprecipitations performed to identify IFIT-binding proteins. Cells were then subject to LS/MS-MS analysis on a Orbitrap Fusion and data analysed in Maxquant v1.5.7.4

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293et Cell

SUBMITTER: Edward Emmott

LAB HEAD: Trevor Sweeney

PROVIDER: PXD007584 | Pride | 2018-03-16

REPOSITORIES: Pride

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IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA.

Fleith Renata C RC Mears Harriet V HV Leong Xin Yun XY Sanford Thomas J TJ Emmott Edward E Graham Stephen C SC Mansur Daniel S DS Sweeney Trevor R TR

Nucleic acids research 20180601 10

Interferon-induced proteins with tetratricopeptide repeats (IFITs) are highly expressed during the cell-intrinsic immune response to viral infection. IFIT1 inhibits translation by binding directly to the 5' end of foreign RNAs, particularly those with non-self cap structures, precluding the recruitment of the cap-binding eukaryotic translation initiation factor 4F and ribosome recruitment. The presence of IFIT1 imposes a requirement on viruses that replicate in the cytoplasm to maintain mechanis ...[more]

PMID: 29554348

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