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Cellular signaling underlying GSIS in pancreatic β-cells

ABSTRACT: In the present study, we investigated signaling pathways associated with GSIS in freshly isolated rat Islets of Langerhans. Specifically, our aim was to reveal which phosphosites and N-linked sialylated glycosylation sites that were reversible regulated upon brief glucose stimulation and link these to cellular signaling pathways using bioinformatics tools.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Rattus Norvegicus (rat) Mus Musculus (mouse)

TISSUE(S): Pancreatic Islet, Primary Cell, Islet Of Langerhans

SUBMITTER: Taewook Kang

LAB HEAD: Martin R. Larsen

PROVIDER: PXD007689 | Pride | 2017-11-14

REPOSITORIES: Pride

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Characterization of the Molecular Mechanisms Underlying Glucose Stimulated Insulin Secretion from Isolated Pancreatic β-cells Using Post-translational Modification Specific Proteomics (PTMomics).

Kang Taewook T Jensen Pia P Huang Honggang H Lund Christensen Gitte G Billestrup Nils N Larsen Martin R MR

Molecular & cellular proteomics : MCP 20171107 1

Normal pancreatic islet β-cells (PBCs) abundantly secrete insulin in response to elevated blood glucose levels, in order to maintain an adequate control of energy balance and glucose homeostasis. However, the molecular mechanisms underlying the insulin secretion are unclear. Improving our understanding of glucose-stimulated insulin secretion (GSIS) mechanisms under normal conditions is a prerequisite for developing better interventions against diabetes. Here, we aimed at identifying novel signal ...[more]

PMID: 29113996

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