Ontology highlight
ABSTRACT:
INSTRUMENT(S): 6410 Triple Quadrupole LC/MS
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: James Olzmann
LAB HEAD: James Olzmann
PROVIDER: PXD007695 | Pride | 2017-12-22
REPOSITORIES: Pride
Bersuker Kirill K Peterson Clark W H CWH To Milton M Sahl Steffen J SJ Savikhin Victoria V Grossman Elizabeth A EA Nomura Daniel K DK Olzmann James A JA
Developmental cell 20171221 1
Lipid droplet (LD) functions are regulated by a complement of integral and peripheral proteins that associate with the bounding LD phospholipid monolayer. Defining the composition of the LD proteome has remained a challenge due to the presence of contaminating proteins in LD-enriched buoyant fractions. To overcome this limitation, we developed a proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells. Application of this approach to two different ce ...[more]