Dataset Information
Quantitative Proteomics for Xenopus Embryos
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Xenopus Laevis (african Clawed Frog)
SUBMITTER: 
Matthew Sonnett
LAB HEAD: Martin Wuehr
PROVIDER: PXD007915 | Pride | 2018-09-03
REPOSITORIES: Pride
Publications
Quantitative Proteomics for Xenopus Embryos II, Data Analysis.
Methods in molecular biology (Clifton, N.J.) 20180101
The oocytes, embryos, and cell-free lysates of the frog Xenopus laevis have emerged as powerful models for quantitative proteomic experiments. In the accompanying paper (Chapter 13) we describe how to prepare samples and acquire multiplexed proteomics spectra from those. As an illustrative example we use a 10-stage developmental time series from the egg to stage 35 (just before hatching). Here, we outline how to convert the ~700,000 acquired mass spectra from this time series into protein expres ...[more]
