Dataset Information

Polylysine interactome in human cells

ABSTRACT: Polylysine chains are synthesized by ribosomes when a stop codon preceding the polyA tail on the mRNA fails to terminate the translation. To clarify the faith of the polylysine-tagged chains, we analyzed polylysine interactome in the cytosol of human 293T cells. We found an extensive network of interactors involving key complexes of protein quality control, such as TRiC and 26S proteasome. The presence of components of the nuclear transport machinery among polylysine interactors and an enhanced nuclear import suggest a mechanism for compartmentalization of faulty proteins in the cell.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Kidney

SUBMITTER: Giulia Calloni

LAB HEAD: R. Martin Vabulas

PROVIDER: PXD008171 | Pride | 2018-04-26

REPOSITORIES: Pride

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Publications

Polylysine is a Proteostasis Network-Engaging Structural Determinant.

Lang Wei-Han WH Lang Wei-Han WH Calloni Giulia G Vabulas R Martin RM

Journal of proteome research 20180425 5

C-terminal polylysine (PL) can be synthesized from the polyadenine tail of prematurely cleaved mRNAs or when a read-though of a stop codon happens. Due to the highly positive charge, PL stalls in the electrostatically negative ribosomal exit channel. The stalled polypeptide recruits the Ribosome-associated quality control (RQC) complex which processes and extracts the nascent chain. Dysfunction of the RQC leads to the accumulation of PL-tagged proteins, induction of a stress response, and cellul ...[more]

PMID: 29634277

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Project description:Amongst the various different insect groups, there is remarkable diversity in the number and size of wings. However the development of the basic body plan in insects is similar to a large extent. The genes of the hox complex regulate various pathways to bring about the development or modification of different organs. Ubx, a gene of the bithorax hox complex is expressed in the third thoracic segment of insects and is known to specify the fate of wing appendage in that segment.To understand the role of Ubx and how its regulatory mechanism has evolved through the course of evolution we have compared its genome wide targets in different insect orders. The identification of regulatory pathways and the key players Ubx regulates is crucial to understand how it has controlled wing development across insect orders. Our lab has previously identified direct targets of Ubx in Drosophila using ChIP-chip (Agrawal et al, 2011). To further our knowledge on the role of regulation in development and modification of hind wing appendage we have studied the targets in the hind wings of other insects (silk moth; Lepidoptera and honeybee; Hymenoptera) and performed a comparative analysis. We have employed ChIP followed by illumina sequencing to identify the targets of Ubx in developing hind and fore wing buds of Bombyx larvae. This is a first next generation sequencing study in Lepidoptera in an attempt to understand wing development. Chromatin Immunoprecipitation (ChIP) was used to identify genome wide targets bound by Ubx in Bombyx larval wing buds. The experiment to enrich Ubx bound regions was carried out using a Bombyx N terminal-Ubx specific poylclonal antibody raised in Rabbit and purified against a Protein A column to obtain IgG fraction. An Immunoprecipitation (IP) with Normal Rabbit IgG was used as a negative control to eliminate the regions that pertained to non specific binding to an Immunogloubulin. The normalization of both ChIP and IgG was done against sequenced input chromatin. Two replicates of single end 36 bp reads were sequenced using Ilumina for all the three conditions and for both fore and hind wing tissue samples.The peaks common to both the replicates were considered after applying a FDR cutoff.The fore wing target set was used for comparison with the hind wing targets.

Dataset Information

Polylysine interactome in human cells

Publications

Polylysine is a Proteostasis Network-Engaging Structural Determinant.

Similar Datasets

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