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Preservation methods and PBS washing affect the AML proteome

ABSTRACT: In the current study, we have studied the effect of three storage and preparation protocols on two AML-derived cell lines: 1) cryopreservation of viable cells for storage in liquid nitrogen, 2) cell pellet storage in liquid nitrogen and 3) freshly SDS lysed cell lysate stored at -°80. We further studied the effect cell pellet wash with cold PBS had on the AML patient cell lysate, aiming to remove remaining contaminants from FBS and blood.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell, Cell Suspension Culture, Leukocyte

DISEASE(S): Acute Leukemia

SUBMITTER: Elise Aasebo

LAB HEAD: Frode Selheim

PROVIDER: PXD008361 | Pride | 2018-01-19

REPOSITORIES: Pride

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Preservation Method and Phosphate Buffered Saline Washing Affect the Acute Myeloid Leukemia Proteome.

Wangen Rebecca R Aasebø Elise E Trentani Andrea A Døskeland Stein-Ove SO Bruserud Øystein Ø Selheim Frode F Hernandez-Valladares Maria M

International journal of molecular sciences 20180119 1

Acute myeloid leukemia (AML) primary cells can be isolated from peripheral blood, suspended with media containing bovine serum and cryoprotectant, and stored in liquid nitrogen before being processed for proteomic analysis by mass spectrometry (MS). The presence of bovine serum and human blood proteins in AML samples can hamper the identifications of proteins, and thereby reduce the proteome coverage of the study. Herein, we have established the effect of phosphate buffered saline (PBS) washing ...[more]

PMID: 29351208

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