Preservation methods and PBS washing affect the AML proteome

ABSTRACT: In the current study, we have studied the effect of three storage and preparation protocols on two AML-derived cell lines: 1) cryopreservation of viable cells for storage in liquid nitrogen, 2) cell pellet storage in liquid nitrogen and 3) freshly SDS lysed cell lysate stored at -°80. We further studied the effect cell pellet wash with cold PBS had on the AML patient cell lysate, aiming to remove remaining contaminants from FBS and blood.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell, Cell Suspension Culture, Leukocyte

DISEASE(S): Acute Leukemia

SUBMITTER: Elise Aasebo  

LAB HEAD: Frode Selheim

PROVIDER: PXD008361 | Pride | 2018-01-19

REPOSITORIES: Pride