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TMT10-TAILS for monitoring MALT1 substrate cleavage

ABSTRACT: We used TMT-TAILS to monitor MALT1 substrate cleavage in normal EBV B lymphocytes stimulated with PMA/ionomycin to evaluate kinetic profiles of MALT1 inhibitors

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Cell Culture

SUBMITTER: Theo Klein

LAB HEAD: Christopher M. Overall

PROVIDER: PXD008421 | Pride | 2018-10-02

REPOSITORIES: Pride

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			Action	DRS
	tails_1_ms2.pep.xml	Pepxml
	tails_1_ms2.raw	Raw
	tails_1_spsms3.pep.xml	Pepxml
	tails_1_spsms3.raw	Raw
	tails_1_universal.pep.xml	Pepxml

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TMT10-TAILS for monitoring MALT1 substrate cleavage

Project description:We used TMT-TAILS to monitor MALT1 substrate cleavage in normal EBV B lymphocytes stimulated with PMA/ionomycin to evaluate kinetic profiles of MALT1 inhibitors

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TMT10-TAILS investigation of the proteolytic landscape in B cell activation

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MALT1 protease activity controls the expression of inflammatory genes in keratinocytes upon Zymosan stimulation

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TMT10-TAILS investigation of the proteolytic landscape in B cell activation

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Project description:We used microarray to monitor the differentially expresed genes during Jurkat T cell activaiton. Jurkat T cells with control shRNA or IKKe shRNA were treated with solvent or PMA and ionomycin for 3 h, and then RNA was extracted and applied to microarray analysis

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