Dataset Information

Monocyte responses to Al(OH)3 adjuvant

ABSTRACT: Monocytes, derived from three different donors, were stimulated with the vaccine adjuvant Al(OH)3 for 24 and 48 hours. Proteins were isolated and equal protein amounts were labeledusing TMT(6). We found that Al(OH)3 activates various pathways related to the innate immune response.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Blood

SUBMITTER: Sietske Kooijman

LAB HEAD: Hugo Meiring

PROVIDER: PXD008452 | Pride | 2018-03-27

REPOSITORIES: Pride

ACCESS DATA

Publications

Novel identified aluminum hydroxide-induced pathways prove monocyte activation and pro-inflammatory preparedness.

Kooijman Sietske S Brummelman Jolanda J van Els Cécile A C M CACM Marino Fabio F Heck Albert J R AJR Mommen Geert P M GPM Metz Bernard B Kersten Gideon F A GFA Pennings Jeroen L A JLA Meiring Hugo D HD

Journal of proteomics 20180106

Aluminum-based adjuvants are the most widely used adjuvants in human vaccines. A comprehensive understanding of the mechanism of action of aluminum adjuvants at the molecular level, however, is still elusive. Here, we unravel the effects of aluminum hydroxide Al(OH)<sub>3</sub> by a systems-wide analysis of the Al(OH)<sub>3</sub>-induced monocyte response. Cell response analysis by cytokine release was combined with (targeted) transcriptome and full proteome analysis. Results from this comprehen ...[more]

PMID: 29317357

Similar Datasets

Project description:1α,25(OH)2VD3 is the most active form of VD3 in animals and it plays an important role in regulating mineral metabolism but also reproduction and immunity. Testes are the main reproductive organs of male mammals. In order to study the effect of 1α,25(OH)2VD3 on early testicular development, 140 weaned 21-day old piglets were selected. The piglets were randomly divided into four groups and were fed a commercial diet supplemented with 0, 1, 2 and 4 μg.kg-1 of 1α,25(OH)2VD3, provided as 1α,25(OH)2VD3-glycosides, 60 days after the start of the experiment, at piglet age 82 days, testes were harvested. The morphology and histology of early testicular development were assessed. In addition, the proteomic TMT/iTRAQ labeling technique was used to analyze the protein profile of the testes in each group. Western blotting was used to verify the target of differentially abundant proteins (DAPs). The results showed that of the identified 132715 peptides, 122755 were specific peptides. 7852 proteins, of which 6573 proteins contain quantitative information. Screening for DAPs was focused on proteins closely related to the regulation of the testicular development such as steroid hormone synthesis, steroid biosynthesis, peroxisome and fatty acid metabolism pathways. These results also indicate that 1α,25(OH)2VD3 is involved in the regulation of early testicular development in piglets. At the same time, these findings provide valuable information for the proteins involved in the regulation of testicular development, and help to better understand the mechanisms of 1α,25(OH)2VD3 in regulating the development of piglets’ testes. Significance: The early development of the testes of breeding boars is closely related to their lifetime fertility. Our research identifies potential signaling pathways and differentially abundant proteins associated with testicular development. These findings indicate that plants containing 1α,25(OH)2VD3-glycosides used as a nutritional ingredient may help early testicular development in breeding boars and reveal the regulatory mechanism of 1α,25(OH)2VD3 on testicular development.

Dataset Information

Monocyte responses to Al(OH)3 adjuvant

Publications

Novel identified aluminum hydroxide-induced pathways prove monocyte activation and pro-inflammatory preparedness.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure