Proteomics

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Erythrocytes lacking the Langereis blood group protein ABCB6 are resistant to Plasmodium falciparum malaria parasites


ABSTRACT: Epstein Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B-cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain novel insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8000 cellular and 69 EBV proteins, including >500 plasma membrane proteins, providing temporal views of the lytic B-cell proteome and EBV virome. Our approach revealed EBV-induced remodelling of cell cycle, innate and adaptive immune pathways, including upregulation of the complement cascade and proteasomal degradation of the B-cell receptor complex, conserved between EBV types I and II. Cross-comparison with proteomic analyses of human cytomegalovirus infection and of a Kaposi sarcoma associated herpesvirus immunoevasin identified host factors targeted by multiple herpesviruses. Our results provide an important resource for studies of EBV replication.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte

SUBMITTER: Michael Weekes  

LAB HEAD: Michael Weekes

PROVIDER: PXD008752 | Pride | 2018-10-19

REPOSITORIES: Pride

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Erythrocytes lacking the Langereis blood group protein ABCB6 are resistant to the malaria parasite <i>Plasmodium falciparum</i>.

Egan Elizabeth S ES   Weekes Michael P MP   Kanjee Usheer U   Manzo Jale J   Srinivasan Ashwin A   Lomas-Francis Christine C   Westhoff Connie C   Takahashi Junko J   Tanaka Mitsunobu M   Watanabe Seishi S   Brugnara Carlo C   Gygi Steven P SP   Tani Yoshihiko Y   Duraisingh Manoj T MT  

Communications biology 20180503


The ATP-binding cassette transporter <i>ABCB6</i> was recently discovered to encode the Langereis (Lan) blood group antigen. Lan null individuals are asymptomatic, and the function of ABCB6 in mature erythrocytes is not understood. Here, we assessed ABCB6 as a host factor for <i>Plasmodium falciparum</i> malaria parasites during erythrocyte invasion. We show that Lan null erythrocytes are highly resistant to invasion by <i>P. falciparum</i>, in a strain-transcendent manner. Although both Lan nul  ...[more]

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