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Saccharomyces cerevisiae mitochondrial fractions by LC-MS/MS

ABSTRACT: Investigation into the role of the Saccharomyces cerevisiae ATP-dependent RNA helicase MSS116 in mitochondrial splicing.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Holger Kramer

LAB HEAD: Tobias Warnecke

PROVIDER: PXD008785 | Pride | 2018-04-09

REPOSITORIES: Pride

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Normal mitochondrial function in <i>Saccharomyces cerevisiae</i> has become dependent on inefficient splicing.

Rudan Marina M Bou Dib Peter P Musa Marina M Kanunnikau Matea M Sobočanec Sandra S Rueda David D Warnecke Tobias T Kriško Anita A

eLife 20180323

Self-splicing introns are mobile elements that have invaded a number of highly conserved genes in prokaryotic and organellar genomes. Here, we show that deletion of these selfish elements from the <i>Saccharomyces cerevisiae</i> mitochondrial genome is stressful to the host. A strain without mitochondrial introns displays hallmarks of the retrograde response, with altered mitochondrial morphology, gene expression and metabolism impacting growth and lifespan. Deletion of the complete suite of mit ...[more]

PMID: 29570052

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