Proteomics

Dataset Information

27

Semen of gibel carp


ABSTRACT: In our study, differential male nucleus events and development behaviors were revealed from the fertilized eggs in response to the sperm from males of genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) in gibel carp. When the eggs of maternal fish were fertilized by the sperm from males of GSD, the fertilized egg encountered similar sexual reproduction events and behaviors. However, when the eggs of maternal fish were fertilized by the sperm from males of TSD, a typical process of gynogenesis was observed. To reveal the underlying molecular mechanism of differential sperm nucleus development behaviors in the fertilized eggs, iTRAQ-based quantitative semen proteomics were performed on three semen samples from three males of GSD and three semen samples from three males of TSD respectively.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Carassius gibelio  

TISSUE(S): Semen

DISEASE(S): Not Available

SUBMITTER: Yaojun Zhu  

LAB HEAD: Jian-Fang Gui

PROVIDER: PXD008919 | Pride | 2018-06-14

REPOSITORIES: Pride

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Publications

Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish.

Zhu Yao-Jun YJ   Li Xi-Yin XY   Zhang Jun J   Li Zhi Z   Ding Miao M   Zhang Xiao-Juan XJ   Zhou Li L   Gui Jian-Fang JF  

BMC genomics 20180605 1


BACKGROUND:Coexistence and transition of diverse sex determination strategies have been revealed in some ectothermic species, but the variation between males caused by different sex determination strategies and the underlying mechanism remain unclear. Here, we used the gynogenetic gibel carp (Carassius gibelio) with both genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) strategies to illustrate this issue. RESULTS:We found out that males of GSD and TSD in gibel  ...[more]

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