Dataset Information
Proximity tagging - A proximity-tagging system to identify membrane protein-protein interactions
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: 
Jun Zheng
LAB HEAD: Min ZHUANG
PROVIDER: PXD008933 | Pride | 2018-05-30
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| CD28tailbindingproteins.zip | Other | |||
| ComparisonBioID.zip | Other | |||
| ComparisonPUP-IT.zip | Other | |||
| LQ_HF_20170224_6_3ul_60min_38.raw | Raw | |||
| LQ_HF_20170224_6_3ul_60min_39.raw | Raw |
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Publications
A proximity-tagging system to identify membrane protein-protein interactions.
Nature methods 20180813 9
The communication between cells and between cellular organelles is often controlled by the interaction of membrane proteins. Although many methods for the detection of protein-protein interactions (PPIs) exist, membrane PPIs remain difficult to detect. Here we developed a proximity-based tagging system, PUP-IT (pupylation-based interaction tagging), to identify membrane protein interactions. In this approach, a small protein tag, Pup, is applied to proteins that interact with a PafA-fused bait, ...[more]
