Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: Joao Paulo
LAB HEAD: Joao A. Paulo
PROVIDER: PXD008966 | Pride | 2018-12-04
REPOSITORIES: Pride
Paulo Joao A JA Navarrete-Perea Jose J Erickson Alison R AR Knott Jeffrey J Gygi Steven P SP
Journal of the American Society for Mass Spectrometry 20180418 7
Phosphorylation-mediated signaling pathways have major implications in cellular regulation and disease. However, proteins with roles in these pathways are frequently less abundant and phosphorylation is often sub-stoichiometric. As such, the efficient enrichment, and subsequent recovery of phosphorylated peptides, is vital. Mass spectrometry-based proteomics is a well-established approach for quantifying thousands of phosphorylation events in a single experiment. We designed a peptide internal s ...[more]