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Arabidopsis thaliana anti-all-WRKY pull-downs from whole seedlings

ABSTRACT: The experiments were performed to monitor dynamics in WRKY transcription factor abundancies upon treatment with flg22, a peptide derived from the bacterial flagella. Mock-treated or WT seedlings treated for 2 h with flg22 were used to prepare crude nuclear lysates. Then pull downs were performed with an anti-all-WRKY antiserum to enrich for WRKY transcription factor proteins prior to MS.

OTHER RELATED OMICS DATASETS IN: GSE109149GSE109151GSE109150

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling

SUBMITTER: Iris Finkemeier

LAB HEAD: Iris Finkemeier

PROVIDER: PXD008971 | Pride | 2018-07-30

REPOSITORIES: Pride

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Principles and characteristics of the Arabidopsis WRKY regulatory network during early MAMP-triggered immunity.

Birkenbihl Rainer P RP Kracher Barbara B Ross Annegret A Kramer Katharina K Finkemeier Iris I Somssich Imre E IE

The Plant journal : for cell and molecular biology 20180908 3

During microbe-associated molecular pattern-triggered immunity more than 5000 Arabidopsis genes are significantly altered in their expression, and the question arises, how such an enormous reprogramming of the transcriptome can be regulated in a safe and robust manner? For the WRKY transcription factors (TFs), which are important regulators of numerous defense responses, it appears that they act in a complex regulatory sub-network rather than in a linear fashion, which would be much more vulnera ...[more]

PMID: 30044528

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