Dataset Information


Online Weak Cation Exchange-HILIC × Reversed Phase LC UVPD-HRMS of intact HeLa Histones

ABSTRACT: Intact HeLa core Histones were analyzed using an online two-dimensional liquid chromatographic separation as well as one dimensional LC (RPLC and WCX-HILIC) coupled with UVPD-MS


ORGANISM(S): Homo sapiens  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Andrea Gargano  

LAB HEAD: Ljiljana Paša-Tolić

PROVIDER: PXD008978 | Pride | 2018-09-19


altmetric image


Increasing the Separation Capacity of Intact Histone Proteoforms Chromatography Coupling Online Weak Cation Exchange-HILIC to Reversed Phase LC UVPD-HRMS.

Gargano Andrea F G AFG   Shaw Jared B JB   Zhou Mowei M   Wilkins Christopher S CS   Fillmore Thomas L TL   Moore Ronald J RJ   Somsen Govert W GW   Paša-Tolić Ljiljana L  

Journal of proteome research 20181008 11

Top-down proteomics is an emerging analytical strategy to characterize combinatorial protein post-translational modifications (PTMs). However, sample complexity and small mass differences between chemically closely related proteoforms often limit the resolution attainable by separations employing a single liquid chromatographic (LC) principle. In particular, for ultramodified proteins like histones, extensive and time-consuming fractionation is needed to achieve deep proteoform coverage. Herein,  ...[more]

Similar Datasets

2014-12-16 | PXD001303 | Pride
2016-08-15 | PXD004630 | Pride
2016-09-13 | PXD003074 | Pride
2017-04-10 | PXD004892 | Pride
2017-11-14 | PXD005424 | Pride
2017-06-23 | PXD005420 | Pride
2016-04-11 | PXD002768 | Pride
2018-05-29 | PXD004166 | Pride
2012-07-30 | E-GEOD-17999 | ArrayExpress
2012-06-30 | E-MTAB-453 | ArrayExpress