Proteomics

Dataset Information

83

Analysis of epididymal sperm maturation by top-down mass spectrometry


ABSTRACT: Spermatozoa Top-down analyses of different epididymal regions to identify endogenous biomolecules.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Sus scrofa domesticus  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Labas Valerie  

LAB HEAD: Valérie Labas

PROVIDER: PXD001303 | Pride | 2014-12-16

REPOSITORIES: Pride

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Publications

Analysis of epididymal sperm maturation by MALDI profiling and top-down mass spectrometry.

Labas Valérie V   Spina Lucie L   Belleannee Clémence C   Teixeira-Gomes Ana-Paula AP   Gargaros Audrey A   Dacheux Françoise F   Dacheux Jean-Louis JL  

Journal of proteomics 20141014


The fertilization ability of male gametes is achieved after their transit through the epididymis where important post-gonadal differentiation occurs in different cellular compartments. Most of these maturational modifications occur at the protein level. The epididymal sperm maturation process was investigated using the ICM-MS (Intact Cell MALDI-TOF MS) approach on boar spermatozoa isolated from four different epididymal regions (immature to mature stage). Differential and quantitative MALDI-TOF  ...[more]

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