Dataset Information

Glycomic and Proteomic changes in aging brain nigrostriatal pathway

ABSTRACT: This study identifies the age related changes in glycan receptors of heparan sulfate (HS) proteoglycan (receptor for rAAV2), and/or N-glycans with terminal galactose (receptor for rAAV9) and proteomics for receptors and co-receptors of adeno-associated virus in the nigro-striatal region of the brain. We analyzed the striatum and substantia nigra for changes in HS, N-glycans and proteomic signatures in young versus aged rat brain nigro-striatum from histological sections. These studies provide insight into age- and brain region-specific changes in glycan receptors and proteomics that will inform design of improved viral vectors for Parkinson’s disease gene therapy.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Rekha Raghunathan

LAB HEAD: Joseph Zaia

PROVIDER: PXD008990 | Pride | 2018-06-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	03062017-RR_SN-Rat-18-rerrun.mgf	Mgf
	03062017-RR_SN-Rat-18-rerrun.mzML	Mzml
	03062017-RR_SN-Rat-18-rerrun.raw	Raw
	03062017-RR_SN-Rat-19-rerrun.mgf	Mgf
	03062017-RR_SN-Rat-19-rerrun.mzML	Mzml

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Publications

Glycomic and Proteomic Changes in Aging Brain Nigrostriatal Pathway.

Raghunathan Rekha R Polinski Nicole K NK Klein Joshua A JA Hogan John D JD Shao Chun C Khatri Kshitij K Leon Deborah D McComb Mark E ME Manfredsson Fredric P FP Sortwell Caryl E CE Zaia Joseph J

Molecular & cellular proteomics : MCP 20180618 9

Parkinson's disease (PD) is a neurological disorder characterized by the progressive loss of functional dopaminergic neurons in the nigrostriatal pathway in the brain. Although current treatments provide only symptomatic relief, gene therapy has the potential to slow or halt the degeneration of nigrostriatal dopamine neurons in PD patients. Adeno-associated viruses (AAV) are vectors of choice in gene therapy because of their well-characterized safety and efficacy profiles; however, although gene ...[more]

PMID: 29915149

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