Serial In-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis

ABSTRACT: Advancement in mass spectrometry has revolutionised the field of proteomics. However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation. Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions. Of particular interest is the brain extracellular matrix (ECM), which has been called the “final frontier” in neuroscience that consists of highly glycosylated proteins. Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) and form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases. Thus, there is a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture. The protocol presented here integrates glycomics and proteomics to analyze multiple classes of biomolecules.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine) Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Brain, Blood Plasma, Articular Cartilage

SUBMITTER: Manveen Sethi  

LAB HEAD: Prof. Joseph Zaia

PROVIDER: PXD017513 | Pride | 2020-04-30

REPOSITORIES: Pride