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Barley seed malting analysis - Temporal analyses of barley malting stages using shot-gun proteomics

ABSTRACT: Quantitative comparison of proteomes generated during germination stages of Barley seeds

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Seed

SUBMITTER: Greg Sabat

LAB HEAD: Dr. Ramamurthy Mahalingam

PROVIDER: PXD009103 | Pride | 2018-07-05

REPOSITORIES: Pride

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Temporal Analyses of Barley Malting Stages Using Shotgun Proteomics.

Mahalingam Ramamurthy R

Proteomics 20180801 15

Malt derived from barley malting is an essential raw material for beer brewing. In this study, we performed the first dynamic proteome survey during barley malting using a gel-free proteomics approach. This entailed in-solution tryptic digestion of precipitated proteins and analysis of peptides by nanoliquid chromatography coupled with tandem mass spectrometry. A total of 1418 proteins were identified from the five malting stages: Steep, 1, 3, 5 days after germination, and end of Kiln. About 900 ...[more]

PMID: 29952130

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