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The proteome of developing barley anthers during meiotic prophase I

ABSTRACT: In the current project, we aimed to discover how the barley anther proteome changes, both quantitatively and qualitatively, during meiotic prophase I when the important process of homologous recombination is taking place. Taking advantage of a recently published barley anther transcriptomic dataset (BAnTr) and a newly developed sensitive mass spectrometry-based approach to analyse barley anther proteome, we have conducted high-resolution mass spectrometry analysis of barley anthers, collected at 6 time points and representing their development from pre-meiosis to metaphase. Each time point was carefully staged using immunocytology. We identified >6400 proteins, including 82 known and putative meiotic proteins, and were able to quantify dynamic variation in the proteome during different stages of barley anther development.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Anther, Plant Cell

SUBMITTER: Runxuan Zhang

LAB HEAD: Robbie Waugh

PROVIDER: PXD026200 | Pride | 2022-04-04

REPOSITORIES: Pride

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The proteome of developing barley anthers during meiotic prophase I.

Lewandowska Dominika D Orr Jamie J Schreiber Miriam M Colas Isabelle I Ramsay Luke L Zhang Runxuan R Waugh Robbie R

Journal of experimental botany 20220301 5

Flowering plants reproduce sexually by combining a haploid male and female gametophyte during fertilization. Male gametophytes are localized in the anthers, each containing reproductive (meiocyte) and non-reproductive tissue necessary for anther development and maturation. Meiosis, where chromosomes pair and exchange their genetic material during a process called recombination, is one of the most important and sensitive stages in breeding, ensuring genetic diversity. Most anther development stud ...[more]

PMID: 34758083

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