The proteome of developing barley anthers during meiotic prophase I

ABSTRACT: In the current project, we aimed to discover how the barley anther proteome changes, both quantitatively and qualitatively, during meiotic prophase I when the important process of homologous recombination is taking place. Taking advantage of a recently published barley anther transcriptomic dataset (BAnTr) and a newly developed sensitive mass spectrometry-based approach to analyse barley anther proteome, we have conducted high-resolution mass spectrometry analysis of barley anthers, collected at 6 time points and representing their development from pre-meiosis to metaphase. Each time point was carefully staged using immunocytology. We identified >6400 proteins, including 82 known and putative meiotic proteins, and were able to quantify dynamic variation in the proteome during different stages of barley anther development.

INSTRUMENT(S):

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Anther, Plant Cell

SUBMITTER: Runxuan Zhang  

LAB HEAD: Robbie Waugh

PROVIDER: PXD026200 | Pride | 2022-04-04

REPOSITORIES: Pride