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Label-free quantitative Proteomics Analysis Reveals Distinct Molecular characteristics in Endocardial Endothelium

ABSTRACT: Endocardial endothelium, which lines the chambers of the heart, is distinct in its origin, structure, and function. However, though functionally very important, no studies at protein level have been conducted so far characterizing endocardial endothelium. In this study, we used endothelial cells from pig heart to investigate if endocardial endothelial cells are distinct at the proteome level. Using a high throughput liquid chromatography-tandem mass spectrometry for proteome profiling and expression, we identified sets of proteins that belong to specific biological processes and metabolic pathways in endocardial endothelial cells supporting its specific structural and functional roles.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Heart, Heart Endothelium

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Aneesh Kumar

LAB HEAD: Dr Abdul Jaleel KA

PROVIDER: PXD009194 | Pride | 2018-06-29

REPOSITORIES: Pride

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Label-free quantitative proteomics analysis reveals distinct molecular characteristics in endocardial endothelium.

Jaleel Abdul A Aneesh Kumar A A Ajith Kumar G S GS Surendran Arun A Kartha Chandrashekaran C CC

Molecular and cellular biochemistry 20180622 1-2

Endocardial endothelium, which lines the chambers of the heart, is distinct in its origin, structure, and function. Characterization studies using genomics and proteomics have reported molecular signatures supporting the structural and functional heterogeneity of various endothelial cells. However, though functionally very important, no studies at protein level have been conducted so far characterizing endocardial endothelium. In this study, we used endothelial cells from pig heart to investigat ...[more]

PMID: 29934862

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